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Published on 28 February 2023

B/CH/20/02 (B20002): Application to release genetically modified wheat strains with improved mildew resistance for experimental purposes

Request authorised march 29, 2021

The current status of the application can be seen at www.protectedsite.ch under "News".

Publication in the Federal Law Gazette (BBl):

Applicant: University of Zurich, Institute of Plant and Microbial Biology

Organism: wheat

Property: improved resistance against mildew

Genetic modification:

- Gene of interest

  • Ubiquitin promoter from maize;
  • Mildew resistance gene: Pm3, Pm8 and Pm17 from wheat and rye; Pm17 and all Pm3 alleles with insertion of a 27 bp long DNA sequence, which codes for the HA epitope tag, before the stop codon; Pm8 with insertion of a 30 bp long DNA sequence, which codes for the myc epitope tag;
  • Native Lr34 termination sequence from wheat.

- Marker gene

  • Ubiquitin promoter from maize;
  • Selection marker gene manA from Escherichia coli;
  • Nopalin synthase termination sequence from Agrobacterium tumefaciens.

Purpose of experiment:

  • Comparing parental lines previously released in 2014-2018 to the stacked lines analysed in the authorized experiment B18001;
  • Clarification of biosafety aspects related to the release of transgenic wheat.

Location of experiment:

  • Protected site of Agroscope at the site of Zurich, Reckenholz, Reckenholzstrasse 191, 8046 Zurich
    Duration of experiment: spring 2021 to autumn 2023

Legal basis:

  • Gene Technology Act (GTA)
  • Release Ordinance (RO)

Decision:

Monitoring: